Journal: PLoS ONE

Article Title: Effect of progesterone on Smad signaling and TGF-β/Smad-regulated genes in lung epithelial cells

doi: 10.1371/journal.pone.0200661

Figure Lengend Snippet: A549 cells were incubated with TGF-β1 (10 ng/mL) and progesterone (10 μg/mL). qPCR of CTGF mRNA was performed after 12 h ( A ) and western blotting analysis after 24 h ( B ). Relative mRNA levels of CTGF were calculated by normalizing signals to detected GAPDH mRNA. Differences compared with untreated cells were calculated. Means + SD of at least n = 3 independent experiments are shown. In B , a representative immunoblot of CTGF and β-actin of n = 3 independent experiments is shown. ** p < 0.01 compared with control cells; ## p < 0.01 compared with cells treated with TGF-β1.

Article Snippet: Membranes were blocked in 5% bovine serum albumin and incubated with primary antibodies recognizing CTGF (ab6992; Abcam, Cambridge, UK), transgelin (sc-50446; Santa Cruz Biotechnology, Santa Cruz, CA), anti-Smad2/3-P (kind gift from Dr. C.-H. Heldin, Ludwig Institute for Cancer Research, Uppsala, Sweden), and β-actin (926–42212; LI-COR Inc., Lincoln, NE, USA), followed by staining with an horseradish peroxidase conjugated goat anti rabbit IgG (Thermo Fisher Scientific).

Techniques: Incubation, Western Blot

Journal: PLoS ONE

Article Title: Effect of progesterone on Smad signaling and TGF-β/Smad-regulated genes in lung epithelial cells

doi: 10.1371/journal.pone.0200661

Figure Lengend Snippet: A549 cells were incubated with TGF-β1 (10 ng/mL) and progesterone (10 μg/mL). Promoter analysis ( A ) and qPCR of transgelin ( TAGLN ) mRNA ( B ) were performed after 12 h, and western blotting analysis ( C ) after 72 h. Relative mRNA levels of TAGLN were calculated by normalizing signals to detected GAPDH mRNA ( B ) and compared with untreated cells. Means + SD of at least n = 3 independent experiments are shown. In C , a representative immunoblot of transgelin and β-actin of n = 3 independent experiments is shown. ** p < 0.01 and *** p < 0.001 compared with control cells; # p < 0.05, ## p < 0.01, and ### p < 0.001 compared with cells treated with TGF-β1.

Article Snippet: Membranes were blocked in 5% bovine serum albumin and incubated with primary antibodies recognizing CTGF (ab6992; Abcam, Cambridge, UK), transgelin (sc-50446; Santa Cruz Biotechnology, Santa Cruz, CA), anti-Smad2/3-P (kind gift from Dr. C.-H. Heldin, Ludwig Institute for Cancer Research, Uppsala, Sweden), and β-actin (926–42212; LI-COR Inc., Lincoln, NE, USA), followed by staining with an horseradish peroxidase conjugated goat anti rabbit IgG (Thermo Fisher Scientific).

Techniques: Incubation, Western Blot

Journal: BMC Cardiovascular Disorders

Article Title: Sevoflurane postconditioning attenuates cardiomyocytes hypoxia/reoxygenation injury via PI3K/AKT pathway mediated HIF-1α to regulate the mitochondrial dynamic balance

doi: 10.1186/s12872-024-03868-1

Figure Lengend Snippet: Antibodies used in western blot

Article Snippet: β-actin , Sino Biological , 100,166-MM10 , 1/15,000.

Techniques: Western Blot, Concentration Assay

Journal: Nucleic Acids Research

Article Title: Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway

doi: 10.1093/nar/gkab445

Figure Lengend Snippet: Functional impairment of cell migration suppression for NKILA lacking CAR-N. ( A ) Wound healing in cell migration assay in MCF-7 cells treated with TGF-β. ( B ) Quantification of cell migration at the indicated time points relative to 0 h ( n = 3), *** P < 0.001, ** P < 0.01. ( C ) Western blot showing the relative expression of E-cadherin and vimentin; β-actin is used as the loading control.

Article Snippet: Antibodies targeted to SRSF1 (ProteinTech 12929–2-AP), SRSF7 (ProteinTech 11044-1-AP), DHX9 (ProteinTech 17721-1-AP), HNRNPC (ProteinTech 11760-I-AP), PRPF8 (ProteinTech 11171-I-AP), SRSF9 (ProteinTech 17926-I-AP), U2AF2 (Santa Cruz-48804), E-cadherin (CST-14472), vimentin (ProteinTech 10366–1-AP), and β-actin (Origene-TA811000), all used at a 1:1000 dilution, and to tubulin (Sigma T9026; 1:10 000 dilution), were used for western blotting.

Techniques: Functional Assay, Migration, Cell Migration Assay, Western Blot, Expressing